The acid-soluble nucleotides of codling (Gadus callarias) muscle

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Abstract
The acid-soluble nucleotides in the skeletal muscle of rested and exercised codling have been separated by a formate elution system on anion-exchange resin, identified and estimated. The 5[image]-mono-, 5[image]-di- and 5[image]-tri-phosphates of adenosine, guanosine and uridine were found. Of the cytidine nucleotides the 5[image]-monophosphate and of the inosine nucleotides the 5[image]-diphosphate and triphosphate were absent. Uridine 5[image]-diphosphate sugar-transferring coenzymes and an unidentified cytidine nucleotide were also detected. Over 80% of the major component of the nucleotide fraction of rested muscle, adenosine 5[image]-triphosphate, was converted into inosine 5[image]-monophosphate during severe exercise. The absence of inosine 5[image]-di- and 5[image]-tri-phosphate may indicate a difference in nucleotide metabolism from that in higher vertebrates.