ESTABLISHMENT OF A HUMAN LEIOMYOSARCOMA CELL LINE

  • 1 January 1977
    • journal article
    • research article
    • Vol. 37  (3) , 658-664
Abstract
A cell line designated SKN was established from the human uterine leiomyosarcoma of a 52 yr old female. The cell line grew well and serial passages were successively done 82 times within 12 mo. Monolayer cultured cells revealed anaplastic and pleomorphic features, and they multipled rapidly without contact inhibition. EM studies revealed myofibrils but no virus-like particles, while chromosomal studies showed that all cultured cells were hyperploid, the modal number was 112 and marker chromosomes were present. Cells were transplanted into an immune depressed hamster cheek pouch and produced a histological leiomyosarcoma resembling the original tumor.