Effects of 6-chloro-6-deoxysugars on glucose oxidation in rat spermatozoa

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Abstract
6-Chloro-6-deoxyfructose or 6-chloro-6-deoxyglucitol (> 90 .mu.mol/kg per day), 6-chloro-6-deoxyglucose or 6-chloro-6-deoxymannose (> 120 .mu.mol/kg per day) and 6-chloro-6-deoxygalactose (> 300 .mu.mol/kg per day) all had an antifertility action in the male rat when given by mouth. Spermatozoa from the infertile rats were unable to oxidize glucose. This effect was always produced by a lower dose than the antifertility effect and the threshold dose for the 2 effects varied in a parallel fashion between the different 6-chloro-6-deoxysugars. Glucose oxidation appeared to be inhibited at the triose phosphate isomerase or glyceraldehyde 3-phosphate dehydrogenase reaction. These effects of 6-chloro-6-deoxysugars are similar to those of .alpha.-chlorohydrin. The activities of the glycolytic enzymes were greatly in excess of the observed flux through the pathway and high concentrations of G-6-P and triose phosphate accumulated in rat spermatozoa incubated with 2 mM-D-glucose.