Universal solid supports for the synthesis of oligonucleotides with terminal 3′-phosphates
Open Access
- 1 January 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 17 (18) , 7149-7158
- https://doi.org/10.1093/nar/17.18.7149
Abstract
The preparation of two types of supports based on controlled pore glass (CPG) is presented. These supports are compatible with established phosphoroamidite chemistry of oligonucleotides synthesis giving rise to an oligonucleotide with terminal 3′-phosphate function during final deprotection. CPG was modified with: (i) methacrylic acid derivatives and 2-mercaptoethanol (1) or (ii) aminoalkylsilane, succinic anhydride and benzidine (2). Support 2 can be also used for the synthesis of partially protected oligonucleotide 3′-phosphatesKeywords
This publication has 16 references indexed in Scilit:
- CHEMICAL-REACTIONS IN NUCLEIC-ACID DUPLEXES .4. CYANOGEN-BROMIDE AS AN EFFICIENT REAGENT IN CONDENSATION OF OLIGODEOXYRIBONUCLEOTIDES1987
- Synthesis and characterization of oligodeoxyribonucleotides containing terminal phosphates. NMR, UV spectroscopic and thermodynamic analysis of duplex formation of [d(pGGAATTCC)]2, [d(GGAATTCCp)]2and [d(pGGAATTCCp)]2Nucleic Acids Research, 1987
- Simple and rapid procedure for synthesis of deoxynucleoside 3'-2-cyanoethyl-N,N-diisopropyl-amino phosphites.1987
- Use of ribonucleosides as protecting groups in synthesis of polynucleotides with phosphorylated terminalsBiochemistry, 1984
- Polymer support oligonucleotide synthesis XVIII1.2): use ofβ-cyanoethyi-N,N-dialkylamino-/N-morpholino phosphoramidite of deoxynucleosides for the synthesis of DNA fragments simplifying deprotection and isolation of the final productNucleic Acids Research, 1984
- A NOVEL TYPE OF DNA MODIFICATION - SITE SPECIFIC INTRODUCTION OF 3'-5'-PYROPHOSPHATE INTERNUCLEOTIDE BONDS1984
- Rapid synthesis of long-chain deoxyribooligonucleotides by the N-methylimidazolide phosphotriester methodNucleic Acids Research, 1983
- DNA-like duplexes with repetitions. III. Efficient template-guided chemical polymerization of d(TGGCCAAGCTp)Nucleic Acids Research, 1981
- Synthesis of 3'-phosphates of diribonucleoside monophosphates via phosphotriester intermediatesNucleic Acids Research, 1976
- Studies on Polynucleotides. XLIII.1 The Synthesis of Deoxyribopolynucleotides Containing Repeating Dinucleotide Sequences2,3Journal of the American Chemical Society, 1965