Kinetic Studies on the Major form of Aldehyde Reductase in ox Kidney: a General Kinetic Mechanism to Explain Substrate-Dependent Mechanisms and the Inhibition by Anticonvulsants

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Abstract
The inhibition of the major form of ox kidney aldehyde reductase (AR 1) by sodium barbitone revealed linear mixed kinetics. This behaviour is distinct from the non-linear intercept effect we reported for valproate [Daly and Mantle (1982) Biochem. J. 205, 381]. 4-Carboxybenzaldehyde exhibits partial uncom petitive substrate inhibition. These results are discussed in terms of a model that involves nucleotide induced isomerization and an additional flux (with some substrates and inhibitors) through an enzyme. nucleotide. substrate/inhibitor ternary complex.