Use of two-colour flow cytometry to assess killing ofGiardia muristrophozoites by antibody and complement
- 1 January 1989
- journal article
- research article
- Published by Cambridge University Press (CUP) in Parasitology
- Vol. 99 (2) , 199-203
- https://doi.org/10.1017/s0031182000058649
Abstract
Summary: A two-colour flow cytometry technique was used to assess killing ofGiardia muristrophozoites by rabbit anti-trophozoite antibodies and complement. Binding of rabbit antibody to trophozoites and killing of trophozoites were documented by flow cytometry, after incubating the organisms with fluorescein-conjugated anti-rabbit IgG and propidium iodide (PI). Percentages of PI+(dead) trophozoites ranged from >80%, after incubation with rabbit antiserum and complement, to Giardia-infected mammals can killGiardiatrophozoites.Keywords
This publication has 25 references indexed in Scilit:
- Enzyme‐linked immunosorbent assay for Giardia‐specific IgA in mouse intestinal secretionsParasite Immunology, 1988
- Mechanism of killing of Giardia lamblia trophozoites by complement.Journal of Clinical Investigation, 1987
- Killing of Giardia lamblia by human milk is mediated by unsaturated fatty acidsAntimicrobial Agents and Chemotherapy, 1986
- Antigiardial activity of the bile salt-like antibiotic sodium fusidateJournal of Antimicrobial Chemotherapy, 1986
- Direct and antibody dependent cell mediated cytotoxicity against Giardia lamblia by splenic and intestinal lymphoid cells in mice.Gut, 1986
- Fluorogenic Substrate Detection of Viable Intracellular and Extracellular Pathogenic ProtozoaScience, 1985
- The sensitivity of Giardia intestinalis to drugs in vitroJournal of Antimicrobial Chemotherapy, 1984
- Factors promoting in vitro excystation of Giardia muris cystsTransactions of the Royal Society of Tropical Medicine and Hygiene, 1984
- Inhibition of clonal growth of Giardia lamblia and Entamoeba histolytica by metronidazole, quinacrine, and other antimicrobial agentsJournal of Antimicrobial Chemotherapy, 1981
- Purification of Giardia Muris Trophozoites by Using Nylon Fiber ColumnsThe American Journal of Tropical Medicine and Hygiene, 1980