Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis of Purified Casein Fractions Treated with Milk-Clotting Enzymes
Open Access
- 1 June 1982
- journal article
- research article
- Published by American Dairy Science Association in Journal of Dairy Science
- Vol. 65 (6) , 899-901
- https://doi.org/10.3168/jds.s0022-0302(82)82288-1
Abstract
Acid casein was fractionated into .alpha.s-, .beta.- and .kappa.-casein and was treated separately with Mucor miehei and Endothia parasitica enzymes and calf chymosin. The enzyme treated casein fractions were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and the electrophoretic patterns were compared with those of untreated casein fractions. All 3 enzymes showed proteolytic activity, but E. parasitica enzyme degraded .alpha.s-casein the most.This publication has 11 references indexed in Scilit:
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