N-FORMYLMETHIONYL-LEUCYL-[H-3]PHENYLALANINE BINDING, SUPEROXIDE RELEASE, AND CHEMOTACTIC RESPONSES OF HUMAN-BLOOD MONOCYTES THAT REPOPULATE THE CIRCULATION DURING LEUKAPHERESIS

  • 1 January 1983
    • journal article
    • research article
    • Vol. 62  (4) , 918-923
Abstract
Human blood monocytes comprise 2 subpopulations: one migrates to the chemoattractant, fMet-Leu-Phe, and has saturable binding sites for this peptide; the other does not migrate and exhibits little peptide binding. To determine if expression of binding sites was a function of monocyte maturation, human subjects were depleted of blood monocytes by leukapheresis so that the circulation was repopulated by monocytes released from the bone marrow. Pre- and postleukapheresis monocytes were then compared for fMet-Leu-[3H]Phe binding, superoxide generation and chemotactic responses. No significant differences in peptide binding curves were found, suggesting that receptor expression was stable over the maturational span represented by these 2 groups of cells. There apparently are 2 distinct lineages of monocytes with respect to expression of receptors for fMet-Leu-Phe. The number of chemotactically responsive cells immediately postleukapheresis was 1/2 the control. This was a transient state; monocyte responses were normal 3 h after termination of leukapheresis, suggesting that they rapidly become functionally mature.

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