The nuclease specificity of the bacteriophage øX174 A*protein
- 1 January 1981
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 9 (3) , 545-562
- https://doi.org/10.1093/nar/9.3.545
Abstract
The A* protein of bacteriophage phi X174 is a single-stranded DNA specific nuclease. It can cleave phi X viral ss DNA in many different places. The position of these sites have been determined within the known phi X174 nucleotide sequence (1). From the sequences at these sites it is clear that the A* protein recognizes and cleaves at sites that show only partial homology with the origin of RF DNA replication in the phi X DNA. Different parts of the origin sequence can be deduced that function as a signal for recognition and cleavage by the A* protein. We conclude that different parts within the DNA recognition domain of the A* protein are functional in the recognition of the origin sequence in single-stranded DNA. The existence of different DNA recognition domains in the A* protein, and therefore also in the A protein, leads to a model that can explain how the A protein performs its multiple function in the phi X174 DNA replication process (2).Keywords
This publication has 22 references indexed in Scilit:
- A new method for sequencing DNA.Proceedings of the National Academy of Sciences, 1977
- Nucleotide sequence of bacteriophage φX174 DNANature, 1977
- Isolation and characterization of the protein coded by gene A of bacteriophage phiX174 DNA.Proceedings of the National Academy of Sciences, 1976
- ‘Single-stranded’ DNA from φX174 and M13 is cleaved by certain restriction endonucleasesNature, 1975
- Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresisBiochemistry, 1975
- Identification of a X174 coded protein involved in the shut-off of host dna replicationBiochemical and Biophysical Research Communications, 1975
- Isolation and Function of the Gene A Initiator of Bacteriophage ϕX 174, A Highly Specific DNA EndonucleaseProceedings of the National Academy of Sciences, 1974
- Two Proteins of Gene A of ϕX174Nature New Biology, 1973
- Formation of the parental replicative form DNA of bacteriophage φX174 and initial events in its replicationJournal of Molecular Biology, 1971
- Mutants of bacteriophage S13 blocked in infectious DNA synthesisJournal of Molecular Biology, 1966