Simplified Way to Cultivate Chick Kidney Cells and Maintain the Culture without Serum
- 25 September 1959
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 130 (3378) , 793
- https://doi.org/10.1126/science.130.3378.793
Abstract
Chick kidney fragments were easily dispersed after incubation in trypsin solution for 1 hour at room temperature. The centrifuged cells were resuspended in Melnick's growth medium, diluted to 100 ml for each pair of kidneys, and seeded at 1 ml per tube. The cultures were maintained for 7 days or longer in the medium modified by replacing the serum with tryptose.Keywords
This publication has 2 references indexed in Scilit:
- TISSUE CULTURE TECHNIQUES AND THEIR APPLICATION TO ORIGINAL ISOLATION, GROWTH, AND ASSAY OF POLIOMYELITIS AND ORPHAN VIRUSESAnnals of the New York Academy of Sciences, 1955
- Monolayer Tissue Cultures I. Preparation and Standardization of Suspensions of Trypsin-Dispersed Monkey Kidney Cells.Experimental Biology and Medicine, 1954