Cortisone on Oxygen Consumption of Granulation Tissue from the Rabbit

Publisher Website
Google Scholar
Abstract
Young, male, white rabbits, weighing 2.5-3.5 kg., were divided into 3 groups relative to the manner in which the dermis was treated: (a) untreated injured, (b) cortisone-treated injured, and (c) untreated uninjured (control) animals. Granulation tissue was produced by sterile removal of a 9 cm.2, depilated, dorsal skin to a depth of 0.035-0.050 in., by means of a Brown Electric Dermatome. Treated animals received 25 mg. cortisone acetate/ day starting 3 days prior to injury. All animals were sacrificed 6 days after the operation. The respiration of these tissues was detd. by the Warburg technique. The tissue was studied in Krebs-phosphate soln. (pH 7.35) at 37.5[degree]C. The results for the previously designated groups were expressed as QO2 (-mm.3 O2/hr./mg. dry wt.): a) - 1.34[plus or minus] 0.06, b) -0.45[plus or minus] 0.03, c) -0.27 [plus or minus] 0.02. Thus (a) gave an increase of 396% and (b) 66% when compared with their controls (c). Cortisone given under these exptl. conditions depressed the respiration of granulation tissue by 66%. These results suggest that cortisone exerts an anti-respiratory effect on granulation tissue. Since this tissue is composed of various cell types, it may be suggested that cortisone may inhibit the growth (anabolic) processes in one or more of the component cells.