CYTOSTATIC ACTIVITY OF INVITRO ACTIVATED HUMAN ADHERENT CELLS AGAINST HUMAN-TUMOR CELL-LINES

  • 1 January 1980
    • journal article
    • research article
    • Vol. 71  (1) , 73-79
Abstract
Human adherent cells from peripheral blood were cultured with immunostimulant, BCG, yeast cell wall or streptococcal preparation (OK 432), for 3 days and the cytostatic activity of the adherent cells on human tumor cells was examined. The cells cultured in the presence of an immunostimulant exhibited increased phagocytic activity and the number of phagocytosed sheep red blood cells (sRBC) per cell increased. Adherent cells cultured without the immunostimulant showed slight cytostatic activity of 8-20%. HD-10 cells, derived from [human] Hodgkin''s disease, and QG-K and QG-U cells derived from [human] uterine cervical cancer were more susceptible than HeLa [human cervical carcinoma] cells to the adherent cells activated by OK 432 [Picibanil] or yeast cell wall. Relationship between population doubling time and susceptibility to the cytostatic effect mediated by the activated adherent cells was not observed. The supernatant from the activated adherent cells was also effective in inhibiting DNA synthesis of the rapidly proliferating target cells, HD-10 cells and HeLa cells; the proliferation of the other 2 cell lines was enhanced. The effect of activated adherent cells on tumor cell proliferation and its relation to cytostasis were examined.