Involvement of acis-element that binds an H2TF-l/NFxB like factor(s) in the virus-induced interferon-β gene expression
- 1 January 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 17 (9) , 3335-3346
- https://doi.org/10.1093/nar/17.9.3335
Abstract
Interferon-.beta. (IFN-.beta.) gene is transcriptionally activated following virus infection of various cell types such as fibroblasts. In the previous studies, regulatory DNA sequences that mediate the virus-induced transcriptional activation have been identified within the 5''-flanking region (up to around-117 respect to the CAP site) of the human IFN-.beta. gene. The sequences contain binding sites (-100 to -61) for a transcriptional activator, IRF-1, the gene of which is also virus-inducible. In the present study, we focused on an additional cis-element, located between the IRF-1 binding sites and TATA box. Interestingly, the element coincides with the previously identified elements for the transcription factors H2TF-1 and NF.vkappa.B. The element, when tandemly repeated, functions in activating the distal gene expression in either constitutive or virus-inducible manner depending on the cell type. The results suggest the importance of cooperation between IRF-1 and H2TF-1/NF.vkappa.B-like factor in the maximal IFN-.beta. gene induction.Keywords
This publication has 29 references indexed in Scilit:
- Involvement of a common transcription factor in the regulated expression of IL-2 and IL-2 receptor genesInternational Immunology, 1989
- Regulated expression of a gene encoding a nuclear factor, IRF-1, that specifically binds to IFN-β gene regulatory elementsCell, 1988
- The same inducible nuclear proteins regulates mitogen activation of both the interleukin-2 receptor-alpha gene and type 1 HIVCell, 1988
- Reversible silencing of enhancers by sequences derived from the human IFN-α promoterCell, 1987
- Interferon-β gene regulation: Tandemly repeated sequences of a synthetic 6 bp oligomer function as a virus-inducible enhancerCell, 1987
- The SV40 enhancer is composed of multiple functional elements that can compensate for one anotherCell, 1986
- The human β-interferon gene enhancer is under negative controlCell, 1986
- A 46-nucleotide promoter segment from an IFN-α gene renders an unrelated promoter inducible by virusCell, 1985
- Competition for cellular factors that activate metallothionein gene transcriptionNature, 1984
- Inhibition of SV40 replication in simian cells by specific pBR322 DNA sequencesNature, 1981