RNA synthesis in isolated nuclei: in vitro initiation of adenovirus 2 major late mRNA precursor.
- 1 January 1979
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 76 (1) , 160-164
- https://doi.org/10.1073/pnas.76.1.160
Abstract
The RNA produced in vitro by incubating nuclei from [human cervical carcinoma] HeLa cells infected with adenovirus serotype 2 was analyzed. Adenovirus-specific RNA is produced at a linear rate for up to 2.5 h. Hybridization analysis of RNA produced in nuclei isolated 18 h after infection indicated that transcription begins at the late promoter at map position 16.5. Sequence analysis of the 5''termini of the in vitro transcripts showed that this system initiates RNA chains de novo at the correct promoter and that the 5''terminus is capped.This publication has 23 references indexed in Scilit:
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