The separation of the mycobactins from Mycobacterium smegmatis by using high-pressure liquid chromatography
- 1 December 1978
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 175 (3) , 853-857
- https://doi.org/10.1042/bj1750853
Abstract
The family of mycobactins from M. smegmatis were resolved into 7 fractions by high-pressure liquid chromatography. This separation was by virtue of the differences in length and character of the long acyl substituents as shown by GLC of the methyl esters of the isolated fatty acids from the fractions. As TLC could also resolve the individual mycobactin fractions, it too must rely on the same differences to effect separation. As the lengths of the acyl chains were modulated by the growth conditions, a specific range of acyl groups may not be needed for mycobactin to function. This technique provides a simple means of rapidly characterizing crude mycobactins from all mycobacteria.This publication has 6 references indexed in Scilit:
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