Two components of type III protein kinase C with different substrate specificities and a phospholipid‐dependent but Ca2+‐inhibited protein kinase in rat brain
- 5 June 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 249 (2) , 324-328
- https://doi.org/10.1016/0014-5793(89)80651-9
Abstract
The activities of rat brain protein kinase C isoenzymic fractions separated by hydroxyapatite chromatography were measured with histone H1 or the oligopeptide Ala-Ala-Ala-Ser-Phe-Lys-Ala-Lys-Lys-amide as substrates. The oligopeptide was a better substrate than histone H1 for nearly all of the protein kinase C fractions. Two subtractions of type III isoenzyme were resolved (IIIa and IIIb); type IIIb was characterized by a very low histone kinase activity compared to its peptide kinase activity. In some brain extracts a phospholipid-dependent but Ca2+-inhibited protein kinase was also observed which was eluted from the hydroxyapatite column between type II and III isoenzymes of protein kinase CKeywords
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