Identification of the structures of multiple subspecies of protein kinase C expressed in rat brain
- 15 June 1987
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 217 (2) , 227-231
- https://doi.org/10.1016/0014-5793(87)80668-3
Abstract
Rat brain protein kinase C purified to apparent homogeneity [(1986) Biochem. Biophys. Res. Commun. 135, 636–643] was resolved into three distinct fractions, type I, II and III, upon chromatography on a hydroxyapatite column connected to high-performance liquid chromatography. Comparison of each fraction with the four subspecies of protein kinase C, that were separately expressed in COS cells transfected by the respective cDNAs, α, βI,βII an d γ, identified the primary structures of these three fractions of protein kinase C. Type I corresponded to the enzyme encoded by the γ-sequence; type II was a mixture of the two subspecies determined by the βI- and βII-sequences; and type III had the structure encoded by the α-sequence. The structures and properties of these subspecies of protein kinase C were similar to each other.Keywords
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