Properties of aflatoxin—DNA adducts formed by photoactivation and characterization of the major photoadduct as aflatoxin—N7-guanine
- 1 April 1990
- journal article
- research article
- Published by Oxford University Press (OUP) in Carcinogenesis: Integrative Cancer Research
- Vol. 11 (4) , 519-527
- https://doi.org/10.1093/carcin/11.4.519
Abstract
DNA adducts were formed by microsomal and photoactivation, using nick-translated DNA labelled with 14C in each of the DNA bases [3H]AFB1 and [3H] AFB2. DNA adducts were analysed by HPLC of DNA hydrolysates, and were characterized as double labelled peaks with specific retention times. The only AF—DNA adducts which were detected in signincant amounts were guanine adducts, irrespective of the type of aflatoxin used or the mode of its activation. No stable adduct with adenine, cytcshe or thymine was detected. UV spectra, proton NMR spectroscopy and mass spectrometry are consistent with the notion that the major AFB1—DNA photoadduct is the N7-guanine adduct. This report provides direct evidence for the existence of ahtoxin photoadducts formed on DNA.Keywords
This publication has 1 reference indexed in Scilit:
- Photoactivated aflatoxins are mutagens to Salmonella typhimurium and bind covalently to DNA in vitroCarcinogenesis: Integrative Cancer Research, 1981