Amperometric amino acid electrodes

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Abstract
Substrate specificity, the linear measurement range, and the lifetime of monoenzyme electrodes were determined using L‐glutamate oxidase, L‐lysine oxidase, and tyrosinase immobilized in gelatin. These sensors were applied to the analysis of protein hydrolyzates. By coimmobilizing the three enzymes, an electrode responding to a group of amino acids was obtained. Its analytical performance was compared with that of an L‐amino acid oxidase sensor. The net charge of the immobilization matrix influenced the relative sensitivities of the amino acids.
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