Production of carrier proteins for insulin‐like growth factors (IGFs) by rat osteoblastic cells Regulation by IGF I and cortisol
- 27 February 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 244 (2) , 328-332
- https://doi.org/10.1016/0014-5793(89)80556-3
Abstract
A bone‐derived rat cell line, PyMS, releases IGF I and IGF carrier proteins which are similar to those found in rat serum. Western blot analysis of culture media conditioned by hormone‐treated cells shows that growth hormone and IGF I stimulate and cortisol inhibits production of IGF carrier proteins in vitro. A glycosylated carrier protein species of 49‐42 kDa is closely related to the subunits of the growth hormone‐dependent carrier protein complex found in rat serum. In addition, rhIGF I rapidly induces a 32 kDa, non‐glycosylated IGF‐binding protein whose accumulation is markedly increased by cortisol.Keywords
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