Instability in tyrR Strains of Plasmids Carrying the Tyrosine Operon: Isolation and Characterization of Plasmid Derivatives with Insertions or Deletions

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Abstract
The transformation of tyrR strains of Escherichia coli with multicopy plasmids which carry the tyrosine operon gave rise to modified plasmids with either insertions or deletions. The effect of each of these insertions or deletions was to decrease the level of expression of this operon. It is proposed that plasmid instability arose as a direct consequence of the metabolic effects of an overproduction of the enzymes coded for by the tyrosine operon. The results have significant implications for the cloning of genes that are repressed by the product of a regulatory gene. Since the predominant plasmid modification observed was the insertion of an IS1 [insertion sequence 1] element near the regulatory region of the tyrosine operon, the results also suggest a role for IS1 elements in the regulationof gene expression.