EFFECTS OF BIOGENIC-AMINES ON FORMATION OF ADENOSINE-3',5'-MONOPHOSPHATE IN HUMAN THYROID SLICES

  • 1 January 1976
    • journal article
    • research article
    • Vol. 23  (4) , 319-325
Abstract
The effects of various concentrations of biogenic amines on the formation of cyclic AMP and their interactions with other thyroid stimulators were investigated in human thyroid slices from normal and Graves'' disease. Most biogenic amines had stimulatory effects to some extent. Among the biogenic amines tested, histamine was the most potent thyroid stimulator, norepinephrine and serotonin, the intermediate in terms of cyclic AMP formation. The effect of histamine was almost as potent as TSH [thyrotropin] in thyroid slices from Graves'' disease. This stimulatory effect of histamine was blocked by metiamide, a histamine H2-receptor antagonist, but not by chlorpheniramine, a histamine H1-receptor antagonist. The effect of norepinephrine was completely inhibited by propranolol, but not by phentolamine. Polyphloretin phosphate did not inhibit norepinephrine- or histamine-induced cyclic AMP formation, while it significantly depressed cyclic AMP formation induced by prostaglandin E2. The maximal effect of histamine was additive to that of TSH. Biogenic amines, histamine and norepinephrine, in particular, probably have the thyroid receptors different from that of TSH or prostaglandin E2 and could play an important role in thyroid physiology.