Isolation of Q nucleoside precursor present in tRNA of an E.coli mutant and its characterization as 7-(cyano)-7-deazaguanosine
Open Access
- 1 January 1978
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 5 (11) , 4215-4224
- https://doi.org/10.1093/nar/5.11.4215
Abstract
One of the E. coli mutants selected for deficiency of modified nucleoside Q was found to contain an analogue of Q and normal guanosine in place of Q. The analogue of Q, designated as preQo, was isolated on a large scale from purified tRNATYr containing preQo. The structure of preQo was determined to be 7-(cyano)-7-deazaguanosine by comparison of its ultraviolet absorption spectra, thin-layer chromatographic mobility and mass spectrum with those of synthetic material.Keywords
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