HPLC Determination of α-Amino Acids in Phytoplanktonic Marine Cells
- 1 November 1990
- journal article
- research article
- Published by Taylor & Francis in International Journal of Environmental Analytical Chemistry
- Vol. 41 (3-4) , 173-176
- https://doi.org/10.1080/03067319008027359
Abstract
A procedure for the quantitative determination of 17 amino acids in a marine matrix using HPLC is reported. Pre-column derivatization with o-phthalaldehyde, separation on C18-bonded silica with phosphate buffer (pH 7.2)-acetonitrile as eluent and fluorescence detection have been used. The good variation coefficient (average 2% with working curves in real matrix) and the low detection limit (1-5 fmoles) make the procedure suitable for the determination of total or free amino acids in matrix cultures.Keywords
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