5-bromodeoxyuridine-DNA strand symmetry and the repair of photolytic breaks in Chinese hamster cell chromosomes.
- 1 November 1976
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 73 (11) , 3905-3909
- https://doi.org/10.1073/pnas.73.11.3905
Abstract
The black light sensitivities of Chinese hamster lung (CHL) cells containing BrdUrd-bromodeoxyuridine-DNA of defined composition were studied. Cesium chloride equilibrium gradient centrifugation provides estimates both of the percent thymidine replacement by BrdUrd and of the symmetry (unifilar vs. bifilar) of BrdUrd incorporation into the chromosomal DNA duplexes. Radiation damage to BrdUrd-substituted CHL cell DNA and its repair in situ also were assessed by alkaline sucrose gradient sedimentation. Animal cell sensitivities to visible light (300-400 nm wavelength) depend markedly upon the symmetries of BrdUrd-substitution within the cells DNA. Cells that contain only unifilar BrdUrd-DNA are resistant to black light, whereas cells that contain bifilar BrdUrd-DNA are extremely photosensitive. The former cell populations repair single-stranded nicks (breaks in phosphodiester bonds) in their DNA within 24 h of irradiation; the latter cell populations, however, are not able to repair light-induced, double-stranded breaks in their DNA.This publication has 18 references indexed in Scilit:
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