FURTHER STUDIES ON THE METABOLISM OF GLUCOSE AND THE FORMATION OF CORTICOSTEROIDS IN VITRO

Abstract
Glucose, adenosine, uridine, and reduced triphosphopyridine-nucleotide (TPNH) significantly increase the effect of corticotrophin (ACTH) on the formation of steroids by rat adrenals in vitro. This effect of glucose is inhibited by 2-deoxyglucose. Inhibition is overcome by TPNH. During the incubation of adrenal fragments, but not of intact glands, TPN-dependent dehydrogenases leak into the medium.