INCREASED AGGLUTINABILITY OF BLADDER CELLS BY CONCANAVALIN-A AFTER ADMINISTRATION OF CARCINOGENS

  • 1 January 1980
    • journal article
    • research article
    • Vol. 40  (6) , 2006-2009
Abstract
The agglutination by concanavalin A of isolated epithelial cells of the rat bladder was examined after in vivo treatment of rats with various bladder carcinogens for 1 wk. The carcinogens tested were N-butyl-N-(4-hydroxybutyl)nitrosamine dibutylnitrosamine, N-[4-(5-nitro-2-furyl)-2-thiazolyl]formamide, 2-acetylaminofluorene, 2-naphthylamine, benzidine, N-methyl-N-nitrosourea and cyclophosphamide. They were given to male Wistar rats orally, s.c., intravesically or i.p. As negative controls, the effects of administration of 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide, dimethylnitrosamine, N-methyl-N''-nitro-N-nitrosoguanidine and surgical implantation of glass beads in the bladder were also tested. One week after the start of treatment, epithelial cells were isolated from the bladder by sonication and agglutination of the isolated cells with concanavalin [Con] A was assayed. The observed agglutinabilities of isolated cells were closely correlated with the reported bladder carcinogenicities of these chemicals in rats. Con A agglutination of bladder cells should be a useful rapid in vivo mammalian system for screening bladder carcinogens.