Direct measurement of octanol-water partition coefficients by high-pressure liquid chromatography

Abstract
A technique is presented for the direct measurement of octanol-water partition coefficients by HPLC for use in lung analysis. The method involves running solutes in octanol-saturated water as the mobile phase against water-saturated octanol entrained on an inert support. Log P correlates linearly with log tc for a number of standards. The measurable range in log P (so far) is -0.03 to +3.7. A critical review of chromatographic methods in Hansch analysis is given.

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