2-Aminopurine Allows Interspecies Recombination by a Reversible Inactivation of the Escherichia coli Mismatch Repair System
- 15 February 2003
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 185 (4) , 1459-1461
- https://doi.org/10.1128/jb.185.4.1459-1461.2003
Abstract
2-Aminopurine treatment of Escherichia coli induces a reversible phenotype of DNA mismatch repair deficiency. This transient phenotype results in a 300-fold increase in the frequency of interspecies conjugational recombination with a Salmonella enterica serovar Typhimurium Hfr donor. This method can be used for the generation of biodiversity by allowing recombination between diverged genes and genomes.Keywords
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