Dominant and recessive mitogen‐nonproliferative variants of 3T3 cells

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Abstract
We have previously isolated 3T3 cell variants unable to respond to specific mitogens. In this report we analyze the dominant and/or recessive nature of these variants. (1) Two independently isolated EGF nonproliferative variants are unable to bind EGF. Hybrids between 3T3R5 cells (thymidine kinase deficient, ouabain‐resistant) and these variants express EGF receptors; the “EGF receptorless” phenotype of these variants is recessive. (2) Hybrids between these two variants do not bind EGF; they are defective in a common, non‐complementing function. (3) A TPA nonproliferative 3T3 variant is also recessive; hybrids with 3T3R5 mount a mitogenic response to TPA. (4) In contrast a fourth variant, which can neither bind labeled EGF nor respond to TPA, is dominant for both characteristics. Hybrids between this latter variant and 3T3R5 can neither bind EGF nor mount a mitogenic response to TPA.