REACTION OF CELLS WITH ANTI-ACTIN SERA IN RELATION TO AMOUNT OF CELLULAR ACTIN

  • 1 January 1977
    • journal article
    • research article
    • Vol. 28  (3) , 512-516
Abstract
The staining pattern of anti-actin sera on various cells [mouse thymocytes, lymphoblastoid cells and mouse fibroblast L cells] smeared on glass was compared to the relative amount of cellular actin estimated by SDS[sodium dodecyl sulfate-]-polyacrylamide-gel electrophoresis with subsequent scanning of the gel. Although the cells showed a varying stainability, the actin content was fairly constant. The staining differences reflected changes in the organization of cellular actin rather than actual differences in the amount of actin.