Antibodies to N6-(Δ2-isopentenyl) adenosine and its nucleotide: interaction with purified tRNAs and with bases, nucleosides and nucleotides of the isopentenyladenosine family
- 1 January 1978
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 5 (9) , 3439-3455
- https://doi.org/10.1093/nar/5.9.3439
Abstract
The interaction of antibodies directed toward N6-(delta2-isopentenyl)adenosine, i6Ado, or its nucleotide with related bases, nucleosides, nucleotides and purified tRNAs is described. The selectivity of the antibody preparation was tested in inhibition experiments utilizing a sensitive radioimmunoassay to quantitate the binding of [3H]i6Ado to the antibody. Purified tRNAs containing various modified nucleosides adjacent to the 3'-end of the anticodon were tested to provide information about the selectivity of the antibody preparation toward nucleotides in this position of the tRNA chain. Antibodies directed against the nucleotide hapten were used to purify tRNAs which contain i6Ado and to quantitate the amount of that nucleotide. The same order of selectivity was expressed whether the nucleotides were free or in a tRNA molecule. Interaction of the antibody with compounds from the i6Ado family demonstrated dominance of the hydrophobic isopentenyl group and the importance of positional differences of modifications.Keywords
This publication has 19 references indexed in Scilit:
- Ribosome structure: localization of N6,N6-dimethyladenosine by electron microscopy of a ribosome-antibody complex.Proceedings of the National Academy of Sciences, 1977
- An improved method for the separation and quantitation of the modified nudeosides of transfer RNANucleic Acids Research, 1976
- Studies on human tRNA I. The rapid, large scale isolation and partial fractionation of placenta and liver tRNANucleic Acids Research, 1975
- Evidence for an unusual immunological response to isopentenyladenosine in rabbitsFEBS Letters, 1974
- Immunologic studies on nucleic acids and their components II. Reversible inhibition of anti-nucleoside antibodies in aqueous pyridine and its application in antibody purificationBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1974
- [40] Preparation of tRNA's and aminoacyl-tRNA synthetases from Bacillus subtilis cells at various stages of growth and sporesPublished by Elsevier ,1974
- Detection of Inosine-containing Transfer Ribonucleic Acid Species by Affinity Chromatography on Columns of Anti-Inosine AntibodiesJournal of Biological Chemistry, 1973
- Immunologic studies on nucleic acids and their components. I. An analysis of the specificity of anti-deoxyadenylate antibodies by a membrane-binding techniqueBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1973
- Identification and isolation of ovalbumin-synthesizing polysomes. I. Specific binding of 125 I-anti-ovalbumin to polysomes.1972
- The Purification and Properties of Ribonucleic Acid from Wheat Germ*Biochemistry, 1963