Copper-induced binding of cellular factors to yeast metallothionein upstream activation sequences.
- 1 January 1989
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 86 (1) , 65-69
- https://doi.org/10.1073/pnas.86.1.65
Abstract
Copper-inducible transcription of the yeast metallothionein gene (CUP1) occurs by means of cis-acting upstream activation sequences (UAS) and trans-acting cellular factors. We have used a high-resolution chromosomal foot-printing technique to detect the interaction of cellular factors with UASCUP1. Our results demonstrate that one or more cellular factors bind to UASCUP1 in a copper-inducible fashion. This copper-inducible binding is enhanced in a yeast strain that harbors several copies of the positive regulatory gene ACE1 and is not detectable in yeast cells that contain a nonfunctional (ace1-.DELTA.1) locus. The correlation between yeast metallothionein gene activation and copper-inducible occupation of UASCUP1 sequences suggests that the binding of metallothionein transcriptional regulatory factors to cis-acting control sequences is copper-inducible.This publication has 20 references indexed in Scilit:
- Promoters, activator proteins, and the mechanism of transcriptional initiation in yeastCell, 1987
- Chemistry and Biochemistry of MetallothioneinPublished by Springer Nature ,1987
- METALLOTHIONEINAnnual Review of Biochemistry, 1986
- Mammalian Metallothionein Is Functional in YeastScience, 1986
- Metallothioneins: Proteins in search of functionCell, 1985
- Copper metallothionein of yeast, structure of the gene, and regulation of expression.Proceedings of the National Academy of Sciences, 1984
- Primary structure and transcription of an amplified genetic locus: the CUP1 locus of yeast.Proceedings of the National Academy of Sciences, 1984
- Transformation in yeast: Development of a hybrid cloning vector and isolation of the can1 geneGene, 1979
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977
- Gene Control of Copper-Sensitivity in SaccharomycesNature, 1955