Similar effects of adenovirus E1A and glucocorticoid hormones on the expression of the metalloprotease stromelysin

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Abstract
The stomelysin (sml) gene encodes a secreted protease which degrades components of the extracellular matrix. Transformation of NRK49F cells by the EIA region of adenovirus (Ad) type 5 or 12 reduces sml RNA levels, whereas various growth factors or EJras-mediated transformation stimulate sml gene expression in these cells. Nuclear run-on experiments show that AdEIA, growth factors and EJras act on sml gene expression at the level of transcription. Although the sml gene is strongly suppressed in AdEIA-transformed cells, treatment with growth factors of transfection of EJras still causes a rise in sml mRNA levels, indicating that EIA does not block the induction mechanism itself. The effect of glucocorticoid hormones on sml gene expression is very similar to that of AdEIA, in that mRNA levels are lowered without affecting the induction phenomenon. The similarity may provide a clue to the mechanism by which AdEIA represses cellular gene activity.