DETECTION AND PARTIAL CHARACTERIZATION OF CROHNS-DISEASE TISSUE SPECIFIC PROTEINS RECOGNIZED BY CROHNS-DISEASE SERA

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  • 1 January 1984
    • journal article
    • research article
    • Vol. 55  (1) , 41-48
Abstract
To identify immunoreactive proteins in Crohn''s disease (CD) tissue, intestinal tissues were examined from 8 patients with CD, 7 patients with ulcerative colitis (UC) and 4 normal colon specimens from patients with colon carcinoma. Tissues were homogenized in 0.05 M Tris-buffered saline containing 2 mM phenyl-methyl-sulfonyl fluoride and the cell free supernatants were incubated with sera from 8 patients with CD, 5 with UC and 5 normal volunteers. Immune complexes formed in vitro were precipitated with pansorbin, washed with several detergents and analyzed by SDS[sodium dodecyl sulfate]-polyacryalmide gel electrophoresis. When CD tissue was immunoprecipitated with CD sera, 3 major proteins of 160,000, 120,000 and 110,000 daltons were detected. Partial precipitated with polyethylene glycol followed by protein A-Sepharose 4B chromatography. Affinity gel chromatography and autoradiographic studies with purified serum IgG from CD patients further confirmed the presence of these immunoreactive proteins in CD tissue extracts. When similiarly examined, these proteins were absent from UC and control tissue extracts after incubation with CD, UC or control sera. CD tissue contains several proteins which are specifically recognized by CD patients'' sera. Characterization of these immunoreactive proteins may provide an important lead in understanding the pathogenesis of CD.