DNA-Dependent RNA-Directed Protein Synthesis In Vitro , IV. Peptide Analysis of an In Vitro and In Vivo ϕX174 Structural Protein
- 1 September 1970
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 67 (1) , 13-17
- https://doi.org/10.1073/pnas.67.1.13
Abstract
An antibody-precipitable structural protein from the bacteriophage ϕX174 was purified from an in vitro protein-synthesizing system primed with the replicative DNA of the virus. This protein was trypsin digested in the presence of the differentially labeled analogous protein that was purified from the bacteriophage. The tryptic hydrolysate was subjected to high resolution peptide chromatography which revealed essentially identical patterns for the ϕX174 proteins synthesized in vitro and in vivo .Keywords
This publication has 7 references indexed in Scilit:
- Further evidence on the formation of poliovirus proteinsJournal of Molecular Biology, 1970
- Initiation of φX-174 RF-primed protein with N-formylmethionineBiochemistry, 1970
- Intermediates in the assembly of φX174Journal of Molecular Biology, 1970
- Electrophoretic characterization of φX174-specific proteinsJournal of Molecular Biology, 1969
- DNA-DEPENDENT RNA-DIRECTED PROTEIN SYNTHESIS in vitro , II. SYNTHESIS OF A ϕX-174 COAT PROTEIN COMPONENTProceedings of the National Academy of Sciences, 1969
- DNA-DEPENDENT RNA-DIRECTED PROTEIN SYNTHESIS in vitro , I. EXTENT OF GENOME TRANSCRIPTIONProceedings of the National Academy of Sciences, 1969
- Accelerated automatic chromatographic analysis of peptides on a spherical resinAnalytical Biochemistry, 1966