Synthesis of functional GABAA receptors in stable insect cell lines
- 14 November 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 335 (1) , 61-64
- https://doi.org/10.1016/0014-5793(93)80439-2
Abstract
We have synthesised the β1-subunit of the bovine GABAA receptor in stable, continuous insect (Spodoptera frugiperda) cell lines. A cDNA was integrated randomly into the insect cell genome under control of a baculovirus immediate early (IE-1) gene promoter. Transformed cells were obtained by co-transfection of the insect cells with pIEK 1 .GRβ1, encoding the β1 subunit cDNA, and pIEK1 .neo, encoding the neomycin resistance gene. G-418-resistant clones were selected and expanded into continuous cell lines synthesising functional, GABA-gated, homo-oligomeric chloride channels. These cell lines had significant advantages over the transient baculovirus expression system for the characterisation of receptors using electrophysiological recording techniques.Keywords
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