INVITRO STIMULATION OF BOVINE PERIPHERAL-BLOOD LYMPHOCYTES - EFFECT OF SHORT-TERM STORAGE OF BLOOD PRIOR TO LYMPHOCYTE CULTURE

  • 1 January 1978
    • journal article
    • research article
    • Vol. 39  (2) , 341-344
Abstract
Storage of peripheral blood from Mycobacterium bovis-sensitized cattle from 1-48 h at 4, 22 and 37.degree. C was shown not to markedly alter the lymphocyte blastogenic response to M. bovis-purified protein derivative. Concanavalin [Con] A-induced lymphocyte mitogenic responses were unaffected by storage of blood for 1, 24 or 48 h at 22.degree. C and 37.degree. C; storage of blood for 48 h at 4.degree. C significantly lowered (P < 0.05) mitogenic responses to Con A, as compared with responses to blood kept at 22.degree. C. Mononuclear cell recovery from stored blood at all temperatures was markedly less than that from freshly drawn blood samples. Cell recoveries were most affected by storage of blood at 4.degree. C and 37.degree. C.