The formation of hippuric acid. The influence of benzoate administration on tissue glycine levels

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Abstract
The influence of the administration of benzoate upon the concentration of glycine in various tissues of the rat and the blood of the rabbit was investigated. Free glycine fell in liver and blood but no significant change occurred in kidney, intestine and skeletal muscle, nor was any change in the concentration of liver-protein glycine observed. After the administration of benzoate, the concentration of free glycine in liver and blood fell rapidly and reached an approximately steady low level which was maintained as along as conjugation continued. Change in concentration was independent of the body level of benzoate until very low levels of benzoate were reached. The significance of these results is discussed with reference to the source of the glycine utilized for conjugation. Turnover rate of free glycine in the liver was calculated. Under the experimental conditions described, a substantial proportion of the glycine used for conjugation by the rat was apparently provided via the free glycine of the liver.