Cloning and nucleotide sequences of the linear DNA killer plasmids from yeast
- 11 October 1984
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 12 (19) , 7581-7597
- https://doi.org/10.1093/nar/12.19.7581
Abstract
The linear DNA killer plasmids (pGKLl and pGKL2) isolated from a Kluyveromyces lactis killer strain are also maintained and expressed its killer character in Saccharomyces cerevisiae. After these killer plasraid DNAs isolated from S. cerevfsiae were treated with alkali, four terminal fragments from each plasmid DNAs were cloned separately. Using these and other cloned DNA fragments, the terminal nucleotide sequences of pGKL2 and the complete nucleotide sequence of pGKLl were determined. The inverted terminal repetitions of 202 bp and 182 bp were found in pGKLl and pGKL2, respectively. The pGKLl sequence showed an extremely high A+T content of 73.2% and it contained five large open reading frames. The largest of these open reading frame was suggested to code for a membrane-bound precursor of glycoprotein subunit of the killer toxin.Keywords
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