EFFECT OF POLYENE ANTIBIOTICS ON PROTOPLASTS OF NEUROSPORA CRASSA

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Abstract
The polyene antibiotics nystatin, amphotericin B, and filipin induced shrinkage of Neuro-spora crassa protoplasts stabilized in sucrose. At low concentrations of filipin (5 [mu]g per ml), or higher concentrations of nystatin and amphotericin B (20 [mu]g per ml), shrinkage was followed by swelling and lysis (bursting) of protoplasts. The effect of various parameters, such as metabolic inhibitors, protoplast concentration and polyene concentration, was determined. The available evidence is consistent with the hypothesis that polyene antibiotics act by altering cellular permeability, possibly by reaction with the cell membrane, of sensitive organisms.

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