Identification of a ribosomal protein essential for peptidyl transferase activity.
- 1 March 1975
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 72 (3) , 844-848
- https://doi.org/10.1073/pnas.72.3.844
Abstract
Extraction with 2 M lithium chloride removes a group of proteins (LiC1 SP) from 50S ribosomal subunits. Both the LiC1 SP and the resulting cores, which contain the remaining proteins as well as 5S and 23S RNA, lack peptidyl transferase activity, as measured by the "fragment reaction". Activity can be restored to the LiC1 cores by reconstitution with LiC1 SP under conditions of high temperature and high ionic strength. The LiC1 SP proteins were fractionated by carboxymethyl-cellulose and Sephadex G-100, and the individual fractions were tested by this reconstitution system. Of the 18 ribosomal proteins found in the LiC1 SP, only L16 is essential for reconstitution of peptidyl transferase activity.Keywords
This publication has 24 references indexed in Scilit:
- E. coli 30 S and 50 S ribosomal subparticle components in the localization region of the tRNA acceptor terminusFEBS Letters, 1974
- Identification of a protein at the ribosomal donor-site by affinity labelingBiochemical and Biophysical Research Communications, 1974
- Photoaffinity labeling of 23 S rRNA inEscherichia coli ribosomes with poly(U)‐coded ethyl 2‐diazomalonyl‐Phe‐tRNAFEBS Letters, 1973
- Affinity labelling of ribosomal peptidyl transferase by a puromycin analogueBiochemical and Biophysical Research Communications, 1973
- Irreversible binding of N‐iodoacetylpuromycin to E. coli ribosomesFEBS Letters, 1973
- Chemical modification of the transfer RNA and polyuridylic acid binding sites of Escherichia coli 30 s ribosomal subunitsJournal of Molecular Biology, 1971
- Topography of the Escherichia coli ribosome: II. Preliminary sequence of 50 s subunit protein attack by trypsin and its correlation with functional activitiesJournal of Molecular Biology, 1971
- Ribosomal proteins of Escherichia coli II. Proteins from the 30 s subunitJournal of Molecular Biology, 1968
- Catalysis of peptide bond formation by 50 s ribosomal subunits from Escherichia coliJournal of Molecular Biology, 1967
- The puromycin reaction and its relation to protein synthesisJournal of Molecular Biology, 1964