Nonspecific adsorption of proteins to microplates.
- 1 February 1973
- journal article
- research article
- Vol. 25 (2) , 279-83
Abstract
When a solution of purified adenovirus hexon proteins was diluted in polystyrene microplates without carrier proteins in the diluent, a higher rate of hexon adsorption to the microplates at higher dilutions took place. Addition of gelatin (0.01%), bovine serum albumin (0.01%), or calf serum (0.2%, vol/vol) to the diluent effectively prevented this adsorption. The adsorption to the microplates of adenovirions and measles virions was also prevented by the carrier proteins in the diluent. Certain additional features which distinguish the microtechnique of Takatsy from conventional macromethods are also discussed.This publication has 13 references indexed in Scilit:
- Isolation and a preliminary characterization of temperature-sensitive mutants of adenovirus 12Virology, 1972
- Influence of mixing on dilutions obtained with the microtiter techniqueJournal of Immunological Methods, 1972
- Solid-phase radioimmunoassay of total and influenza-specific immunoglobulin G.1972
- Association of deoxyribonuclease-sensitive material with adenovirus penton aggregates after treatment of infected cell cultures with sodium deoxycholateCanadian Journal of Microbiology, 1971
- Solid phase radioimmunoassay of human β 1C globulinImmunochemistry, 1970
- Fiber-associated incomplete and complete hemagglutinins of adenovirus type 6Archiv für die gesamte Virusforschung, 1969
- Solid-Phase Radioimmunoassay in Antibody-Coated TubesScience, 1967
- The relationship between soluble antigens and the virion of adenovirus type 3 III. Immunological identification of fiber antigen and isolated vertex capsomer antigenVirology, 1967
- ADSORPTION OF PROTEINS BY POLYSTYRENE PARTICLES .I. MOLECULAR UNFOLDING AND ACQUIRED IMMUNOGENICITY OF IGG1967
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951