MEASUREMENT OF THE CARDIAC-OUTPUT AND ITS PERIPHERIC DISTRIBUTION IN RATS BY MEANS OF RADIOACTIVE MICROSPHERES

  • 1 January 1980
    • journal article
    • research article
    • Vol. 36  (3) , 271-278
Abstract
The cardiac output (CO), the blood flow (BF) through several organs and the peripheric resistances were simultaneously determined on uninephrectomized rats by using 2 kinds of microsphere with a theoretical diameter of 15 .mu.m and labeled with 141Ce or 85Sr. The measured diameter of the microspheres was 13.1 .+-. 0.2 .mu.m (n = 500) for the 141Ce-labeled microspheres and 16.2 .+-. 0.3 .mu.m (n = 500) for the 85Sr ones. The difference is statistically significant (P < 0.005). Both kinds of microspheres gave comparable values for the CO and the renal, splenic and heart coronary BF, but dissimilar values for the hepatic and pulmonary BF. The resulting CO (29.0 .+-. 2.4 ml/min per 100 g) is similar to those reported, and with the splenic, coronary and renal BF, correlating the latter closely with value obtained from the p-aminohippuric acid clearance. The microsphere technique seems to be a simple and time-saving method to measure the CO and BF of such organs as kidneys, spleen and heart of the rat.