Establishment of two cell lines from embryonic tissue of the tobacco hornworm,Manduca sexta (L.)
- 1 November 1975
- journal article
- Published by Springer Nature in In Vitro Cellular & Developmental Biology - Plant
- Vol. 11 (6) , 395-399
- https://doi.org/10.1007/bf02616376
Abstract
Two cell lines were derived from primary cultures of embryonic tissue of the tobacco hornworm,Manduca sexta (L.). The cell lines were maintained on hemolymph-free synthetic insect medium. Cytogenetic and immunological identification of the lines were carried out. Techniques for obtaining the line and medium, subculturing and freezing procedures for long-term storage, and the morphological and growth characteristics are described.Keywords
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