Deacetylation of PS-5, a new .BETA.-lactam compound. II. Separation and purification of L- and D-amino acid acylases from Pseudomonas sp. 1158.
- 31 December 1979
- journal article
- research article
- Published by Japan Antibiotics Research Association in The Journal of Antibiotics
- Vol. 33 (6) , 550-555
- https://doi.org/10.7164/antibiotics.33.550
Abstract
L-Amino acid acylase and D-amino acid acylase of Pseudomonas sp. 1158 which converted PS5 to NS5 (deacetylated PS5) were separated and purified by sonication, streptomycin and ammonium sulfate fractionations, DEAE-Sephacel column chromatography and gel filtration. MW and the isoelectric point were estimated to be 75,000 and pI 5.45 for L-amino acid acylase and 100,000 and pI 4.95 for D-amino acid acylase.This publication has 6 references indexed in Scilit:
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