Overexpression of Desulfovibrio vulgaris Hildenborough cytochrome c553 in Desulfovibrio desulfuricans G200
Open Access
- 1 December 1993
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 218 (2) , 293-301
- https://doi.org/10.1111/j.1432-1033.1993.tb18377.x
Abstract
Plasmid pRC41, containing the cyf gene encoding cytochrome c553 from Desulfovibrio vulgaris Hildenborough, was transferred by conjugation from Escherichia coli to Desulfovibrio desulfuricans G200. The structural properties of the purified protein were studied by one‐dimensional and two‐dimensional NMR. A heterogeneity in the folding of the cytochrome isolated from D. vulgaris Hildenborough and from D. desulfuricans G200 was observed for the oxidized form. Temperature, pH and salt‐dependence studies indicated that the heterogeneity does not result from an intermediate in the protein unfolding process, but derives from two conformations which are not in dynamic equilibrium.Keywords
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