First Derivative Spectrophotometric and Colourimetric Determination of Non-Steroidal Antiestrogens

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Abstract
Three methods have been described for the determination of clomiphene citrate and tamoxifen citrate. The first is based on using the first derivative spectra of UV absorption spectra. The second is based on the formation of colored ionpair compounds between the drugs and methyl orange in chloroform and measure the absorbance at 420 nm. The third is based on the reaction of the drugs with citric acid/acetic anhydride reagent and measure the absorbance at 605 nm. The proposed methods are applied for determination of the drugs in tablets. Compared with the pharmacopoeial methods, the proposed methods gave equally accurate (t-test) and precise (f-test) results. The proposed methods are selective and more sensitive than the pharmacopoeial methods.