DNA sequences required for regulated expression of β-globin genes in murine erythroleukaemia cells

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Abstract
We have introduced into murine erythroleukaemia (MEL) cells a series of human globin gene cosmids and two sets of hybrid genes constructed from the human β-globin gene and the human γ-globin or m urine H-2K bml genes. S1-nuclease analysis of the mRNA products from these genes before and after MEL cell differentiation showed that the hum an β-globin gene, but not the human ε- or γ-globin or H-2K bml genes, is induced specifically. Hybrid genes containing hum an β-globin DNA sequences from either 5' or 3' side of the translation initiation site were both inducible. Measurement of the relative rate of transcription showed this induction to be the result of transcriptional activation. We therefore suggest that DNA sequences which regulate β-globin gene expression during MEL differentiation are located both 5' and 3' to the translation initiation site.