The turnover of cytochrome P450b
- 18 October 1982
- journal article
- Published by Wiley in FEBS Letters
- Vol. 147 (2) , 239-242
- https://doi.org/10.1016/0014-5793(82)81050-8
Abstract
The turnover of the heme and apocytochrome moieties of phenobarbital‐inducible microsomal cytochrome P450 (P450b) was investigated. Adult male Sprague—Dawley rats were treated with phenobarbital for 5 days and injected with [35S]methionine and the heme precursor δ‐[3H]‐aminolevulinic acid. P450b was isolated by immunoprecipitation and quantitated by rocket immunoelectrophoresis. The isotope disappearance curves revealed a mean half‐life (T ) of 12.4 h for the heme moiety and a T of 19.1 h for the apoprotein moeity of P450b. The apparently slower catabolic rate of the apoprotein may be due to reutilization of [35S]methionine and does not exclude synchronous turnover of the two moieties. Our data are consistent with the kinetics of the drug‐mediated induction of cytochrome P450b.
Keywords
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